The sequence-specific hybridization of DNA facilitates its use as a {building|developing|creating|constructing} block for designer nanoscale structures and reaction networks that {perform|carry out|execute} computations. {However|Nevertheless|Nonetheless|Even so|On the other hand|Having said that}, the {strong|powerful|robust|sturdy} binding {energy|power} of Watson-Crick base pairing that underlies this specificity also causes the DNA dehybridization {rate|price} to {depend|rely} sensitively on sequence length and temperature. This {strong|powerful|robust|sturdy} dependency imposes stringent constraints {on the|around the} {design|style|design and style} of multi-step DNA reactions, {because|simply because|since|due to the fact|mainly because|for the reason that} a {small|little|tiny|modest|smaller|compact} deviation {from the|in the} optimal {conditions|circumstances|situations} slows down the {process|procedure|method|approach|course of action} {dramatically|significantly|drastically|substantially|considerably}. {Here|Right here} we show how an ATP-dependent helicase, Rep-X, can drive {certain|particular|specific} dehybridization reactions in {designed|developed|created|made} DNA reaction networks at {rates|prices} independent of sequence length, thereby decoupling the {rates|prices} of hybridization and dehybridization. To illustrate this principle, we show that Rep-X extends the {range of|selection of|array of} {conditions|circumstances|situations} {where|exactly where} the primer exchange reaction, which catalytically adds a domain {provided|supplied|offered} by a hairpin template to a DNA substrate, proceeds {rapidly|quickly|swiftly}: {in the|within the|inside the} {strong|powerful|robust|sturdy} substrate-hairpin binding regime, Rep-X expedites the reaction {almost|nearly|virtually|practically|just about|pretty much} {one|1|a single|one particular} hundred-fold. Our {results|outcomes|final results|benefits} {provide|offer|supply|give|present|deliver} an {example|instance} of how ATP consumption can drive {specific|particular|certain|distinct|precise} dehybridization reactions in {designed|developed|created|made} DNA reaction networks and how this consumption {can be|may be|could be|might be|is often|is usually} harnessed to expedite reactions beyond their equilibrium {rates|prices}. Formula of (3R)-3-Methylpyrrolidin-3-ol Methyl 3-fluoro-5-iodo-2-methylbenzoate In stock PMID:23543429
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