Product Name :
Pan-Lactyl-lysine Recombinant Rabbit Monoclonal Antibody [PSH03-74]
Predicted band size :
Observed band size :
Mutiple kDa
Synonyms:
Lactyllysine antibody
Function :
Post-translational modifications (PTMs) represent a crucial means of regulating diverse biological processes and cellular physiology by influencing protein structure and function. Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300. This modification was high in gene promoter regions that lack acetylation and associated with activation of genes expression. As a large number of studies have demonstrated that lysine acylation possessed a diverse range of substrate proteins, however, no systematic analysis has been reported for lysine lactylation.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic Lactyl lysine-containing peptide.
Species reactivity:
Species independent
Verify application:
WB, IHC-P, IF-Cell, FC, Dot Blot
Coupling :
unconjugated
Clone number:
PSH03-74
RRID:
AB_3096498
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Fmoc-His(3-Me)-OH Order Aliquot store at -20℃.(Diacetoxyiodo)benzene Purity Avoid repeated freeze / thaw cycles.PMID:33499659
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Positive control :
HeLa treated with 100mM Lactate sodium for 24 hours cell lysate, human breast cancer tissue, human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue, HeLa cells treated with 100mM Lactate sodium for 24 hours.